About our services
The Histology and Imaging Core offers services and training related to image analysis and image analysis software, to guide investigators in choosing the best methods for presenting their data.
Request Services
Work and training service requests are made via iLab.
View instructions on setting up an iLab account (PDF)
Sample Processing
The Histology and Imaging Core is fully integrated with the UNE鈥檚 Behavior Core and can directly process samples collected by the Behavior Core.
Light Microscopes
- Thorlabs Bergamo II multi-photon microscope equipped with SpectraPhysics Insight X3 dual emission laser source
- 20x, 60x water immersion lenses
- Galvo-galvo and resonant-galvo scan paths for imaging and stimulation
- Olympus VS200 slide scanning microscope
- Fully automated and enclosed with 6-slide sample holder; DAPI, GFP, TRITC, Cy5 filter cubes; Orca Fusion 16-bit sCMOS camera and Excelitas LED excitation source
- Keyence BZ-X710 inverted widefield digital microscope; DAPI, GFP, TRITC, Cy5 filter cubes; 4x plan fluor/0.15, 10x plan fluor/0.3, 20x plan fluor ELWD/0.45, 20x plan apo/0.7, 40x plan fluor/0.5, 40x plan fluor/0.75, 100x oil plan fluor/1.3
- 12 bit monochrome/color CCD
- XY stitching, Z-stack, and multi-point acquisition (can be combined)
- BZ-X analyzer software for image stitching, quantification, and analysis
- Leica DM2500 upright widefield microscope with automated stage; DAPI, Cy2, Cy3, Cy5 filter cubes; 5x plan fluo/0.15, 10x plan fluo/0.3, 20x plan fluo/0.5, 40x plan fluo/0.75, 63x oil plan apo/1.4
- Stereologer unbiased image analysis software
- Leica DFC 365 FX 16-bit 1.4 MP monochrome CCD
- Imi-Tech Nile2 5 MP color CCD
- Nikon E800 upright widefield microscope (x2); DAPI, Cy2, Cy3 filter cubes; 4x plan fluo/0.14, 10x plan fluo/0.3, 40x plan fluo/0.75, 100x oil plan fluo/1.3
- Diagnostic Instruments RT KE Spot 7.2 Color Mosaic CCD
- Nikon E400 dual-headed brightfield microscope
- 2x plan/0.06, 4x plan/0.1, 10x plan/0.25, 20x plan/0.4, 40x plan/0.65
- ImagingPlanet MPX 3.3 MP color CCD
- Leica MZ6 dual-headed stereomicroscope
- 4.0x zoom
- Jenoptik ProgRes CFcool 14-bit cooled CCD
- Leica Stellaris 5 Point Scanning Confocal Microscope
- DMI8 microscope base with epifluorescent light source
- Diode 405, OPSL 488, OBIS 561, and Diode 638 laser lines
- HC PL Fluotar 10x/0.30, HC PL Fluotar 20x/0.40 LWD, HC PL APO 20x/0.70, HC PL APO CS2 40x/1.30 Oil, HC PL APO CS2 63x/1.40 Oil, and HC PL APO CS 63x/1.20 Water objective lenses
- 2 HyD S photon counting detectors
- Software with built in LIGHTNING image deconvolution, stitching, and tile correction
- Stage top incubator
- LifeCanvas SmartSPIM Microscope
- Large imaging chamber compatible with samples ranging in size from organoids to whole mice
- 488, 561, and 647 laser lines
- 1.6x, 3.6x, and 9x objective lenses
- Hamamatsu Orca Fusion CMOS camera with high-speed rolling shutter synchronized with axially swept Gaussian beam laser
Osteomeasure Workstation
- Software tailored for bone histomorphometry
- Touchscreen tracing for labeling bone, osteoid, muscle, and cells
- Olympus BX53 microscope
- Olympus DP74 camera with Pixel Shift technology
- PL APO 2x/0.08, 4x/0.16, 10x/0.20, 20x/0.80, and 40x/0.95 objective lenses
Contact
For general questions or to submit an imaging services request form, email Histology and Imaging Core Manager Peter Caradonna at pcaradonna@une.edu.
Faculty and Professional Staff
Professor
School of Biological Sciences
College of Arts and Sciences
Director, Histology and Imaging Core
Center for Pain Research (COBRE)
Center for Excellence in the Neurosciences
Histology and Imaging Core Manager
Center for Excellence in the Neurosciences